PEG-6000 Induced Precipitation of Proteins from Cold-Acclimated Tenebrio molitor larvae

Authors

  • Aleksandr K. Gulevsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Elena O. Grischenkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Liana I. Relina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Diana V. Tretyak Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

DOI:

https://doi.org/10.15407/cryo25.04.350

Keywords:

cold acclimation, proteins, polyethyleneglycol, precipitation, electrophoresis

Abstract

Spectra of PEG-6000-precipitated proteins from cold-acclimated and non-acclimated Tenebrio molitor larvae were comparatively studied. The composition of precipitated proteins from cold-acclimated and non-acclimated T. molitor larvae was shown as differed qualitatively. In particular, the protein spectra of filtrates from cold-acclimated larvae had proteins with molecular weight of 11 kDa, which were absent in non-acclimated insects. There was observed a significant difference in precipitate content at polyethyleneglycol concentrations of 12% and higher during protein precipitation from homogenates of cold-acclimated and non-acclimated larvae. Proteins from the filtrates of cold-acclimated larvae in the presence of 6% PEG-6000 were precipitated by 44 and 56% greater, than those from non-acclimated species at 22 and 4°C, respectively. The increasing of PEG-6000 concentration from 4 to 6% resulted in appearance in the precipitates from cold-acclimated larvae of proteins with molecular-weight range of 30–42.5 kDa, which were absent in non-acclimated insects.


Probl Cryobiol Cryomed 2015; 25(4):350-358.

Author Biographies

Aleksandr K. Gulevsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Biochemistry of Cold Adaptation

Elena O. Grischenkova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Biochemistry of Cold Adaptation

Liana I. Relina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Biochemistry of Cold Adaptation

Diana V. Tretyak, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Biochemistry of Cold Adaptation

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Published

2015-12-21

How to Cite

Gulevsky, A. K., Grischenkova, E. O., Relina, L. I., & Tretyak, D. V. (2015). PEG-6000 Induced Precipitation of Proteins from Cold-Acclimated Tenebrio molitor larvae. Problems of Cryobiology and Cryomedicine, 25(4), 350–358. https://doi.org/10.15407/cryo25.04.350

Issue

Section

Theoretical and Experimental Cryobiology