Effect of Cryoprotective Solutions and Their Components on Intensity of Lipid Peroxidation and Antioxidant Activity in Leukocytes at Cryopreservation

Authors

  • Andrey N. Khudyakov Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Tatyana V. Polezhaeva Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Oksana O. Zaitseva Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Denis S. Laptev Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Olga N. Solomina Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Andrey A. Kostyaev Kirov Research Institute of Hematology and Blood Transfusion, Kirov

Keywords:

leukocytes, cryoprotectants, cryopreservatives, lipid peroxidation, antioxidant activity

Abstract

Using chemiluminescence method it was shown that after addition of several cryoprotectants 1,2-propane diol (21% initial concentration hereinafter and added 1:1) and dimethyl sulfoxide (8%) to leukocyte suspension the level of LPO and antioxidant activity decreased. Presence of glycerol (7%) and hexa-methylene bis-tetra hydroxyethyl urea (30%) resulted in simultaneous stimulation of LPO and antioxidant systems. Hydroxyethylated starch (5.7%) and gelatine (molecular weight of 16,000; 7.5%) increase LPO. After storage in combined cryoprotective media at –10, –20 and –40°C the leukocytes revealed the activation of antioxidant systems on the background of stable LPO indices, that, probably, contributed to morphofunctional preservation of 75% of cells. Probl Cryobiol Cryomed 2013; 23(1):49–57.

Author Biography

Andrey A. Kostyaev, Kirov Research Institute of Hematology and Blood Transfusion, Kirov

КировÑкий ÐИИ гематологии и Ð¿ÐµÑ€ÐµÐ»Ð¸Ð²Ð°Ð½Ð¸Ñ ÐºÑ€Ð¾Ð²Ð¸ ФМБРРоÑÑии

References

Baraboy V.A. Stress mechanisms and lipid peroxidation. Uspekhi Sovremennoi Biologii 1991; 111 (6): 21–28.

Belous A.M., Grischenko V.I. Cryobiology Kiev: Nauk. Dumka, 1994.

Galchenko S.E., Mamontova A.V., Sandomirsky B.P. Influence of modes of preservation of porcine spleen fragments on intesity of peroxidation. Problems of Cryobiology 1998; (1): 58–61;

Glants S. Medical and biological statistics Moscow: Praktika, 1998.

Gordiyenko A.D., Kudokotseva E.V. Study of functional state of mitochodria in cell suspensions exposed in cryoprotectant solution. Kriobiologiya i Kriomeditsyna 1980; (7): 32–34.

Klebanov G.I., Lyubitsky O.B., Vasil'eva O.V. et al. Antioxidant properties of 3-oxypyridine derivatives: mexidol, emoxipin and proxipin. Problemy Meditsinskoy Khimiyi 2001; 47 (3): 288–300.

Kornienko E.M., Bondarenko V.A. Perspective in using combined cryoprotectants during blood component freezing by one–step method at –196°C. Problems of Cryobiology 2008; 18 (2): 248.

Onischenko E.V., Zinchenko A.V. Study of effect of some permeable cryoprotectants on microsomes using chemiluminiscence method. Problems of Cryobiology 2005; 15 (1): 50–55.

Sandomirsky B.P., Galchenko S.E., Tynynyka L.N. Integrity of adult pig and newborn piglets liver fragments under different cryopreservation conditions. Problems of Cryobiology 2003; (4): 77–84.

Svedentsov E.P., Stepanova E.S., Tumanova T.V. et al. Application of gelatin-based preparations at development of preventive solution for preservation of leukocytes at over-cooling temperature. Bull Eksp Biol Med 2007; 144 (10): 445–447.

Svedentsov E.P., Tumanova T.V., Khudyakov A.N. et al. Integrity of biological membranes of blood nucleated cells at –80°C. Biologicheskie Membrany 2008; 25 (1): 23–29.

Svedentsov E.P., Tumanova T.V., Shcheglova O.O. et al. Freezing leukocytes to –40°C and –20°C under an exponential programme with original cryopreservatives. International Journal of Refrigeration 2006; 29: 369–373.

Downloads

Published

2013-03-15

How to Cite

Khudyakov, A. N., Polezhaeva, T. V., Zaitseva, O. O., Laptev, D. S., Solomina, O. N., & Kostyaev, A. A. (2013). Effect of Cryoprotective Solutions and Their Components on Intensity of Lipid Peroxidation and Antioxidant Activity in Leukocytes at Cryopreservation. Problems of Cryobiology and Cryomedicine, 22(1), 49–57. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/9

Issue

Section

Theoretical and Experimental Cryobiology