Effect of Cryopreservation According Two-Stage Program in High-Molecular Dextran Solutions on Cytomorphological and Functional Properties of Human Cord Blood Cells

Authors

  • Olga Yu. Kozhina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Maksim V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Igor G. Grisha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Nikolay A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

human cord blood, nucleated cells of blood, cryopreservation, high-molecular dextran, two stage freezing

Abstract

Human cord blood (HCB) is a conventional source of stem cells and biologically active substances. Long-term storage of this unique biomaterial is possible under conditions of low temperature bank. In this research we studied the effect of cryopreservation according to two-stage freezing program on cytomorphological and functional characteristics of cells of HCB leukoconcentrate (HCBL). It was shown that after cryopreservation under protection of high molecular dextran, redistribution in cell composition of HCBL occurred due to reduction of granulocytes population, increase of non-differentiated cells and macrophages number. Quite a high resistance to effect of cryopreservation factors had stem and early progenitor cells. There was noted a reduction of total bactericidal activity of HCBL cells and increase of phagocytic activity of macrophages. Probl Cryobiol Cryomed 2013; 23(1):58–65.

Author Biographies

Olga Yu. Kozhina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Maksim V. Ostankov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Igor G. Grisha, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Nikolay A. Bondarovich, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

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Published

2013-03-15

How to Cite

Kozhina, O. Y., Ostankov, M. V., Grisha, I. G., & Bondarovich, N. A. (2013). Effect of Cryopreservation According Two-Stage Program in High-Molecular Dextran Solutions on Cytomorphological and Functional Properties of Human Cord Blood Cells. Problems of Cryobiology and Cryomedicine, 22(1), 58–65. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/10

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

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Copyright (c) 2020 Olga Yu. Kozhina, Maksim V. Ostankov, Igor G. Grisha, Nikolay A. Bondarovich

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