Cryoprotective Efficiency of Some Cryoprotectants Depending on Cooling Rate

Authors

  • N. A. Chernobai Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, cooling rate, testes interstitial cells, permeability, cryoprotectants

Abstract

The survival of testes interstitial cells after freeze-thawing using the cooling rates of 1; 5 and 10 deg/min and glycerol (7%), dimethyl sulfoxide (10%), ethylene glycol (7%) and dimethyl formamide (5%) solutions has been estimated. Using of glycerol provided sufficiently high (up to 75%) cell survival under all the studied cooling rates. Cell survival increased (from 56% upto 83%) if cooling rates were elevated from 1 up to 10 deg/min in the case of other cryoprotectants. Cooling rates about 10 deg/min provided sufficiently high level of post-thaw cell survival.

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Published

2024-05-21

How to Cite

Chernobai, N. A., Gurina, T. M., & Pakhomov, A. V. (2024). Cryoprotective Efficiency of Some Cryoprotectants Depending on Cooling Rate. Problems of Cryobiology and Cryomedicine, 21(3), 273–279. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/125

Issue

Vol. 21 No. 3 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. A. Chernobai, T. M. Gurina, A. V. Pakhomov

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