Study of Release of Biologically Active Compounds from Cord Blood Under Different Conditions of Low-Temperature Impact

Authors

  • Nataliia M. Moisieieva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-9845-2317
  • Ivan I. Shcheniavskyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-8121-2862
  • Olga L. Gorina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-4075-650X
  • Yuliia S. Akhatova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-1536-6924
  • Oleksandr Yu. Semenchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo33.04.250

Keywords:

cryodestruction, cord blood, biologically active substances, low-molecular fraction, low temperatures

Abstract

Here, we have studied the impact of cord blood destruction method on composition of the cord blood-derived low molecular fractions, and compared the cryodestruction with other methods of cell destruction before extracting. Human cord blood was destroyed by rapid or slow freezing / warming, hypotonic lysis and thermal destruction. The obtained substance was used to produce the cord blood fraction (CBF) by multi-stage ultrafiltration and lyophilization. Dry weight, CBF composition and total protein content in them were evaluated by chromatographic profiles (gel permeation and reverse-phase high-performance liquid chromatography). The CBFs, obtained by different techniques for cord blood destruction were established to differ in the content and molecular weights of the components. These findings suggest the possibility to vary the amount and range of low molecular weight compounds in lyophilized cord blood fractions by using low temperatures and combining different regimens of freezing / warming.

 

Probl Cryobiol Cryomed 2023; 33(4): 250–262

Author Biographies

Nataliia M. Moisieieva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Ivan I. Shcheniavskyi, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Olga L. Gorina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Yuliia S. Akhatova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Oleksandr Yu. Semenchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

References

Brock J, Golding D, Smith PM, et al. Update on the role of Actovegin in musculoskeletal medicine: a review of the past 10 years. Clin J Sport Med. 2020;30(1):83-90. CrossRef

Deus IA, Mano JF, Custódio CA. Perinatal tissues and cells in tissue engineering and regenerative medicine. Acta Biomater. 2020; 110: 1-14. CrossRef

Ehrhart J, Sanberg PR, Garbuzova-Davis S. Plasma derived from human umbilical cord blood: Potential cell-additive or cell-substitute therapeutic for neurodegenerative diseases. J Cell Mol Med. 2018; 22(12): 6157-66. CrossRef

Emara AK, Anis H, Piuzzi NS. Human placental extract: the feasibility of translation from basic science into clinical practice. Ann Transl Med [Internet]. 2020 Mar 17 [cited 2021 Sep 20]; 8(5): 156. Available from: https://atm.amegroups.org/article/view/35941/html CrossRef

Fuller BJ, Lane N, Benson EE. Life in the Frozen State. London: CRC Press; 2004. 672 p. CrossRef

Gulevsky OK, Moisieieva NN, Abakumova OS, Shchenyavsky II, Nikolchenko AYu, Gorina OL. inventors; Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, assignee. [Method of obtaining low-molecular fraction from cord blood of cattle]. Ukraine patent № 69652. 2012 May10. Ukrainian.

Komarov FI, Korovkin BF, Menshikov VV. [Biochemical studies in clinic]. Elista: APP Dzhangar; 2001. 216 p. Russian.

Leonel LCPC, Miranda CMFC, Coelho TM, et al. Decellularization of placentas: establishing a protocol. Braz J Med Biol Res [Internet]. 2017 Nov 17 [cited 2021 Sep 20]; 51(1): e6382. Available from: https://www.scielo.br/j/bjmbr/a/Dwy7ZQQvjsSZtJXbL5gXJbP/abstract/?lang=en CrossRef

McIntyre JA, Jones IA, Danilkovich A, et al. The placenta: applications in orthopaedic sports medicine. Am J Sports Med. 2018; 46(1): 234-47. CrossRef

Pogozhykh O, Prokopyuk V, Figueiredo C, Pogozhykh D. Placenta and placental derivatives in regenerative therapies: experimental studies, history, and prospects. Stem Cells Int [Internet]. 2018 Jan 18 [cited 2021 Sep 20]; 2018: 4837930. Available from: https://www.hindawi.com/journals/sci/2018/4837930 CrossRef

Querol S, Samarkanova D. Rapid review: next generation of cord blood banks; transplantation and beyond. Transfusion. 2019; 59(10): 3048-50. CrossRef

Samarkanova D, Rodríguez L, Vives J, et al. Cord blood-derived platelet concentrates as starting material for new therapeutic blood components prepared in a public cord blood bank: from product development to clinical application. Blood Transfus. 2020; 18(3): 208-16. CrossRef

Shabunin SV, Vostroilova GA, Shabanov IE. [Screening of biologically active substances depending on the technological parameters of cryogenic fractionation of the placenta]. Problems of Cryobiology. 2005; 15(3): 306-9. Russian. Full Text

Silini AR, Cargnoni A, Magatti M, et al. The long path of human placenta, and its derivatives, in regenerative medicine. Front Bioeng Biotechnol [Internet]. 2015 Oct 19 [cited 2021 Sep 20]; 3:162. Available from: https://www.readcube.com/articles/10.3389/fbioe.2015.00162 CrossRef

Xia J, Minamino S, Kuwabara K, et al. Stem cell secretome as a new booster for regenerative medicine. Biosci Trends. 2019; 13(4): 299-307. CrossRef

Zhmakin AI. Physical aspects of cryobiology. Physics-Uspekhi. 2008; 51(3): 231-52. CrossRef

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Published

2023-12-30

How to Cite

Moisieieva, N., Shcheniavskyi, I., Gorina, O., Akhatova, Y., & Semenchenko, O. (2023). Study of Release of Biologically Active Compounds from Cord Blood Under Different Conditions of Low-Temperature Impact. Problems of Cryobiology and Cryomedicine, 33(4), 250–262. https://doi.org/10.15407/cryo33.04.250

Issue

Vol. 33 No. 4 (2023): Probl Cryobiol Cryomed

Section

Theoretical and Experimental Cryobiology

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