Effect of Cooling Rate in the Vicinity of Eutectic Temperature Characteristic for Cryoprotectant Aqueous Solution on Post-Thaw Viability of Saccharomyces cerevisiae and Escherichia coli Cells

Authors

  • Tatyana M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Igor P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Anna L. Polyakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, microorganisms, Saccharomyces cerevisiae, Escherichia coli, eutectic temperature range, cooling rate

Abstract

The effect of various cooling rates in the range of eutectic temperatures of aqueous solutions of cryoprotectants (dimethyl sulfoxide, glycerol, polyethylene oxide with molecular mass 1500, 1,2-propane diol) on viability in cell suspesion of Saccharomyces cerevisiae and Escherichia coli was studied. The cell suspensions were cooled in these temperature ranges with controlled (1 and 25 deg/min) and non-controlled (plunging into liquid nitrogen) rates. Application of controlled cooling rates in eutectic temperature range of the studied cryoprotectant solutions was shown to be prospective for increase the post-thaw viability of microorganisms. The highest indices of viability of S. cerevisiae and E. coli were obtained for the cooling rate of 25 deg/min. This tendency was kept for all the studied concentrations of cryoprotective agents (5, 10, 15, 20%). The findings are recommended to be considered when developing the freezing protocols for other biological specimens. Probl Cryobiol Cryomed 2013; 23(1):15-25.

Author Biographies

Tatyana M. Gurina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long-Term Storage of Biological Objects under Low Temperatures and Microbiology

Igor P. Vysekantsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long-Term Storage of Biological Objects under Low Temperatures and Microbiology

Anna L. Polyakova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Low Temperature Preservation

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Published

2013-03-15

How to Cite

Gurina, T. M., Vysekantsev, I. P., & Polyakova, A. L. (2013). Effect of Cooling Rate in the Vicinity of Eutectic Temperature Characteristic for Cryoprotectant Aqueous Solution on Post-Thaw Viability of Saccharomyces cerevisiae and Escherichia coli Cells. Problems of Cryobiology and Cryomedicine, 22(1), 15–25. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/6

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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Copyright (c) 2020 Tatyana M. Gurina, Igor P. Vysekantsev, Anna L. Polyakova

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