Effect of Cryoprotectants on Hypertonic Cryohemolysis Rate of Human Erythrocytes with Modified Cytoskeleton-Membrane Complex

Authors

  • Ya. O. Cherkashina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

hypertonic cryohemolysis, erythrocytes, cryoprotectants, modifiers of protein sulfhydryl groups

Abstract

There was studied the development of hypertonic cryohemolysis of erythrocytes, modified with iodoacetamide (IAA) and pchlormercuribenzoate (p-CMB) in the media containing cryoprotectants (glycerol, 1,2-propane diol, polyethylene glycerol-1500). It has been shown that independently on the presence in the incubation medium of cryoprotective compounds when modifying the protein component of erythrocytes with IAA and p-CMB the sensitivity of erythrocytes to hypertonic cryohemolysis increases. This may be stipulated with the change in conformation of the proteins directly or indirectly involved into adaptation mechanisms of cells to stress effects.

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Published

2012-06-18

How to Cite

Cherkashina, Y. O. (2012). Effect of Cryoprotectants on Hypertonic Cryohemolysis Rate of Human Erythrocytes with Modified Cytoskeleton-Membrane Complex. Problems of Cryobiology and Cryomedicine, 22(2), 126–132. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/73

Issue

Vol. 22 No. 2 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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Copyright (c) 2020 Ya. O. Cherkashina

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