Assessment of Phenotype Characteristics of Fetal Neural Cells after Cryopreservation Using Different Freezing Regimens

Authors

  • Ye. A. Porozhan Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. N. Babenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

fetal neural cells, cryopreservation, stem cells

Abstract

In the paper the effect of three different cryopreservation regimens on subpopulation composition of fetal neural cells (FNCs) of 11 gestation days was analyzed. FNC subpopulations revealed different sensitivity to the effect of physical and chemical factors of cryopreservation, implemented when using these freezing regimens. The fact of higher cryostability of glial and stem cells in biomaterial of heterogenous composition independently on freezing regimen was found. It has been shown that using one of the regimens in general pool of FNCs the number of multipotent progenitor cells with CD133 and nestin phenotype increases due to the death of more differentiated neuron precursors. Selective effect of certain cryopreservation regimens on heterogenous content of FNCs was proved.

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Published

2024-05-21

How to Cite

Porozhan, Y. A., Ostankov, M. V., Babenko, N. N., & Goltsev, A. N. (2024). Assessment of Phenotype Characteristics of Fetal Neural Cells after Cryopreservation Using Different Freezing Regimens. Problems of Cryobiology and Cryomedicine, 22(1), 39–48. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/96

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 Ye. A. Porozhan, M. V. Ostankov, N. N. Babenko, A. N. Goltsev

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