Impact of Biologically Active Complexes of L. rhamnosus GG and S. boulardii After Storage Under Low Temperatures on Biofilm Forming Ability in Corynebacteria
DOI:
https://doi.org/10.15407/cryo31.02.127Ключевые слова:
corynebacteria, biofilm, biologically active complexes, Lactobacillus rhamnosus GG, Saccharomyces boulardii, antibiofilm formation activity, low temperature regimens of storageАннотация
This paper describes the temperature regimens and shelf life of biologically active complexes (structural components and metabolites) of Lactobacillus rhamnosus GG and Saccharomyces boulardii, which have antimicrobial activity and reduce biofi lm formation in pathogenic agents of Corynebacterium spp. Preservation of biological activity of complexes after 6-month storage (observation period) at (−23 ± 1)°C and for 60 days (observation period) under hypothermia at (4 ± 1)°C has been demonstrated. The degree of inhibition of biofi lm formation in pathogenic corynebacteria depended on sensitivity of the Corynebacterium spp. test-culture strain to products of microbial origin. A mixture of lactobacillus and saccharomycete metabolites displayed weak biofi lm formation by toxicogenic strains of corynebacteria (p < 0.05). Our fi ndings testifi ed to a possible use of biologically active substances, stored at 4 and −23°C in designing the L. rhamnosus GG and S. boulardii complexes and in technological processes of their production to prevent the persistence of the diphtheria pathogens.
Probl Cryobiol Cryomed 2021; 31(2): 127–138
Библиографические ссылки
Atlas RM. Handbook of microbiological media. 4 th edition. Boca Raton: CRC Press; 2010. 217 p. CrossRef
Basnakyan IA. [Cultivation of microorganisms with specified properties]. Moscow: Meditsina, 1992. 188 р. Russian.
Batoni G, Maisetta G, Esin S. Antimicrobial peptides and their interaction with biofilms of medically relevant bacteria. Biochim Biophys Acta. 2016; 1858(5): 1044-60. CrossRef
Chen H, Wubbolts RW, Haagsman HP, et al. Inhibition and eradication of Pseudomonas aeruginosa biofilms by host defence peptides. Sci Rep [Internet]. 2018 Jul 11 [cited 2018 Sep 10]; 8: 10446. Available from: https://www.nature.com/articles/s41598-018-28842-8 CrossRef
Dostert M, Belanger CR, Hancock RE, et al. Design and assessment of anti-biofilm peptides: steps toward clinical application. J Innate Immun. 2019; 11: 193-204. CrossRef
Frickmann Н, Klenk C, Warnke P, et al. Infl uence of probiotic culture supernatants on in vitro biofilm formation of staphylococci. Eur J Microbiol Immunol (Bp). 2018; 8(4): 119-27. CrossRef
Gladkih PG. [Importance of microbiofilms in infection pathology of human (Review)]. Vestnik novykh meditsinskikh tekhnologiy. [Internet]. 2015 Feb 16 [cited 2018 Feb 16]; (1): publ. 3-2. Available from:http://www.vnmt.ru/Bulletin/E2015-1/5096.pdf
Isaenko OYu, Knysh OV, Babych EM, et al. [The influence of metabolic products of L. rhamnosus GG on the test culture of staphylococci and corynebacteria]. Bulletin of Problems in Biology and Medicine. 2017; (2): 246-51. Ukrainian.
Isaienko OYu, Knysh OV, Babych YeM, et al. Influence of storage of probiotic broth culture filtrates on their antimicrobial activity. Probl Cryobiol Cryomed. 2017; 27(4): 311-21. CrossRef
Isayenko OYu, Knysh OV, Babych YeM, et al. [Antimicrobial activity of metabolites of S. boulardii against test cultures of Staphylococci and Corynebacteria]. Pharmacology and Drud Taxicology. 2017; 54 (3): 50-5. Ukrainian.
Isayenko OYu, Knysh OV, Babych YeM, et al., inventors; Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, assignee. [Method of obtaining a combination of metabolites of probiotic strains of fungi and bacteria]. Patent of Ukraine N 126603. 2018 Jun 25. Ukrainian.
Isayenko OYu, Knysh OV, Babych YeM, et al., inventors; Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, assignee. [Method for obtaining metabolites of probiotic strains of bacteria]. Patent of Ukraine N 123122. 2017 Feb 12. Ukrainian.
Knysh OV, Isaenko OYu, Babych EM, et al. Antimicrobial activity of bifidobacteria derivatives after storage in a frozen state. Probl Cryobiol Cryomed. 2018; 28(3): 237-48. CrossRef
Kostyukova NN, Bechalo VA. Diphtheria carriage. Epidemiology and Vaccinal Prevention. 2018;17(5):60-70. Russian. CrossRef
Lluch EJ, Garcia V, Kingdon ADH, et al. A simple polymicrobial biofilm keratinocyte colonization model for exploring interactions between commensals, pathogens and antimicrobials. Front. Microbiol [Internet]. 2020 Feb 26 [cited 2020 Jun 20]; 11: 291. Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2020.00291/full CrossRef
Osama DM, Elkhatib WF, Tawfeik АM, et al. Antimicrobial, antibiofilm and immunomodulatory activity of Lactobacillus rhamnosus and Lactobacillus gasseri against some bacterial pathogens. IJBWI. 2017; 6(1): 12-21. CrossRef
Rybalchenko OV, Bondarenko VM, Orlova OG, et al. [Disorganization of biofilms of clinical staphylococcal strains by lactobacillus metabolites]. Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii. 2010; 6: 66-70. Russian.
Somma АD, Moretta A, Canè C, et al. Antimicrobial and antibiofilm peptides. Biomolecules [Internet]. 2020 apr 23 [cited 2021 Mar 10]; 10(4): 652. Available from: https://www.mdpi.com/2218-273X/10/4/652 CrossRef
Stepanović S, Vuković D, Hola V, et. al. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115(8): 891-9. CrossRef
Wu BC, Haney EF, Akhoundsadegh N, et al. Human organoid biofilm model for assessing antibiofilm activity of novel agents. Biofilms and Microbiomes. 2021; 7(1): 5-13. CrossRef
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