Theoretical determination of biophysical parameters for optimising neural cells' three-dimensional structures cryopreservation

Авторы

DOI:

https://doi.org/10.15407/cryo35.02.076

Ключевые слова:

neural cells, spheroids, aggregates, cultivation, filtration coefficients, permeability coefficients, dimethyl sulfoxide, osmotically inactive volume

Аннотация

Probl Cryobiol Cryomed. 2025; 35(2): 76–84

Биографии авторов

Oleksandr Sukach, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

Ihor Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low-Temperature Preservation

Svitlana Vsevolodska, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

Olga Ochenashko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

Svitlana Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low-Temperature Preservation

Библиографические ссылки

Andrews MG, Kriegstein AR. Challenges of organoid research. Annu Rev Neurosci. 2022; 45: 23-39. CrossRef

Bédard P, Gauvin S, Ferland K, et al. Innovative human three-dimensional tissue-engineered models as an alternative to animal testing. Bioengineering (Basel) [Internet]. 2020 September 17 [cited 2025 May 20]; 7(3): 115. Available from: https://www.mdpi.com/2306-5354/7/3/115 CrossRef

Cho AN, Jin Y, An Y, et al. Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids. Nat Commun [Internet]. 2021 Aug 05 [cited 2025 May 20]; 12(1): 4730. Available from: https://www.nature.com/articles/s41467-021-24775-5 CrossRef

Dingle YT, Boutin ME, Chirila AM, et al. Three-dimensional neural spheroid culture: an in vitro model for cortical studies. Tissue Eng Part C Methods. 2015; 21(12): 1274-83. CrossRef

Gordiyenko YeO, Gordiyenko OI, Maruschenko VV, Sakun OV. [Improved model for the passive mass transfer through the cell plasma membrane]. Biophysical Bulletin. 2008; 21(2); 75-80. Ukrainian

Gordiyenko OI, Kovalenko SYe, Kovalenko IF, et al. Theoretical estimation of the optimum cooling rate of a cell suspension at linear freezing modes based on a two-factor theory of cryodamage. CryoLetters. 2018; 39(6): 380-5. PubMed

Gordiyenko YeO, Pushkar NS. [Physical basis for low temperature preservation of cell suspensions]. Кyiv: Naukova dumka; 1994. 140 p. Russian

Ogurtsova VV, Kovalenko SYe, Kovalenko IF, Gordiyenko OI. Determination of osmotically inactive volume of murine enterocytes. Probl Cryobiol Cryomed. 2016; 26(1): 93-7. CrossRef

Petrenko AYu, Sukach AN. Isolation of intact mitochondria and hepatocytes using vibration. Analytical Biochem. 1991; 194(2): 326-9. CrossRef

Reynolds BA, Rietze RL. Neural stem cells and neurospheres - reevaluating the relationship. Nat Methods. 2005; 2: 333- 6. CrossRef

Sukach AN, Liashenko TD, Shevchenko M.V. Properties of isolated neural cells from newborn rat in tissue in vitro. Biotechnologia Acta. 2013; 6(3):63-8. CrossRef

Sukach OM, Vsevolodska SO, Ochenashko OV, Shevchenko MV. Obtaining and characterization of rat fetuses neural cells aggregates/spheroids. Biopolymers and Cell. 2022; 38(2): 93-102. CrossRef

Загрузки

Опубликован

2025-11-23

Как цитировать

Sukach, O., Kovalenko, I., Vsevolodska, S., Ochenashko, O., & Kovalenko, S. (2025). Theoretical determination of biophysical parameters for optimising neural cells’ three-dimensional structures cryopreservation . Проблемы криобиологии и криомедицины, 35(2), 76–84. https://doi.org/10.15407/cryo35.02.076

Выпуск

Раздел

Теоретическая и экспериментальная криобиология