Freezing regimen affects preservation of canine erythrocytes when using dimethyl sulfoxide

Авторы

Ключевые слова:

cryopreservation, cryoprotectant, canine erythrocytes, dimethyl sulfoxide, freezing regimens

Аннотация

Probl Cryobiol Cryomed. 2026; 36(2): 115—121

Биографии авторов

Natalia Yershova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Cryocytology

Tetiana Gurina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Low Temperature Preservation

Olena Nipot, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Cryocytology

Nataliia Shpakova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Cryocytology

Библиографические ссылки

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Denysova OM, Zhegunov GF. Cryopreservation of canine erythrocytes using dimethyl sulfoxide, polyethylene glycol and sucrose. Probl Cryobiol Cryomed. 2021; 31(1): 38-50. CrossRef

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Langer R, Albrecht R, Hempel K, et al. Characterization of 24-hour survival rate and duration of survival of hydroxyethyl starch cryopreserved erythrocytes after autologous transfusion in the dog. Infusionsther Transfusionsmed. 1994; 21 (6): 393-400. PubMed

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Pogozhykh D, Pakhomova Y, Pervushina O, et al. Exploring the possibility of cryopreservation of feline and canine erythrocytes by rapid freezing with penetrating and non-penetrating cryoprotectants. PLoS ONE [Internet]. 2017 Jan 10 [cited 2025 Sept 5]; 12(1): e0169689. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169689 CrossRef

Ulizko PY, Zhegunov GF, Denisova ON. Influence of freeze-thawing with DMSO on integrity and osmotic fragility of erythrocyte from different mammalian species. Problems of Cryobiology. 2011; 21(1): 52-7. Full Text

Valeri CR, Srey R, Tilahun D, et al. The in vitro quality of red blood cells frozen with 40 percent (wt/vol) glycerol at -80 degrees C for 14 years, deglycerolized with the Haemonetics ACP 215, and stored at 4 degrees C in additive solution-1 or additive solution-3 for up to 3 weeks. Transfusion. 2004; 44: 990-5. CrossRef

Wagner CT, Martowicz ML, Livesey SA, et al. Biochemical stabilization enhances red blood cell recovery and stability following cryopreservation. Cryobiology. 2002; 45: 153-66. CrossRef

Zhegunov GF, Denysova ON. [Permeability of mammalian erythrocytes for the molecules of glycerol and DMSO and the level of cellular viability after freezing-thawing]. Reports of the National Academy of Sciences of Ukraine. 2010; (12): 139-43. Russian.

Zhegunov GF, Denysova ON, Zemlianskykh NG. [Cryopreservation and integrity of animal erythrocytes]. Problems of Cryobiology. 2005; 15(3): 566-9. Russian. Full Text

Zhegunov GF, Denysova ON, Zhegunova GP. Blood hypothermic storage and erythrocyte cryopreservation in dogs. Probl Cryobiol Cryomed. 2022; 32(4): 245-55. CrossRef

Zhegunov GF, Nardid OA, editors. [Fundamentals of cryobiology and cryomedicine]. Kharkiv: Brovin AB; 2019. 616 p. Russian.

Опубликован

2026-07-14

Как цитировать

Yershova, N., Gurina, T., Nipot, O., & Shpakova, N. (2026). Freezing regimen affects preservation of canine erythrocytes when using dimethyl sulfoxide. Проблемы криобиологии и криомедицины, 36(2), 115—121. извлечено от https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2194

Выпуск

Раздел

Криоконсервирование биологических систем