Сучасні підходи і перспективи методів ліофілізації ядерних клітин кордової крові людини

Автор(и)

  • Anatoliy Goltsev Інститут проблем кріобіології і кріомедицини НАН України, м. Харків https://orcid.org/0000-0002-5289-5876
  • Olena Lutsenko Інститут проблем кріобіології і кріомедицини НАН України, м. Харків https://orcid.org/0000-0003-3982-9687
  • Lyudmyla Ostankova Інститут проблем кріобіології і кріомедицини НАН України, м. Харків
  • Mykola Bondarovych Інститут проблем кріобіології і кріомедицини НАН України, м. Харків https://orcid.org/0000-0001-7977-0283
  • Maksym Ostankov Інститут проблем кріобіології і кріомедицини НАН України, м. Харків https://orcid.org/0000-0001-8412-5262
  • Larysa Sokil Інститут проблем кріобіології і кріомедицини НАН України, м. Харків https://orcid.org/0000-0002-8702-7374
  • Igor Hrisha Інститут проблем кріобіології і кріомедицини НАН України, м. Харків https://orcid.org/0000-0003-2875-910X
  • Lyudmyla Chernyshenko Інститут проблем кріобіології і кріомедицини НАН України, м. Харків https://orcid.org/0000-0003-4246-1369

DOI:

https://doi.org/10.15407/cryo33.04.227

Ключові слова:

лейкоконцентрат кордової крові людини, ліофілізація, ліопротектори, антиоксиданти

Анотація

В огляді узагальнено дані щодо перспектив застосування ліофілізації як методичного підходу до збереження структурно-функціональних характеристик з метою довгострокового зберігання лейкоконцентрату кордової крові людини (ЛККЛ). Показано переваги методу ліофілізації порівняно з низькотемпературним зберіганням, природнім або тепловим висушуванням. Розглянуто питання впливу фізико-хімічних факторів, які реалізуються на етапі ліофілізації, на структурно-функціональні характеристики клітин ЛККЛ. Обговорюється важливість таких чинників, як-от концентрація клітин, умови зберігання, залишкова вологість у забезпеченні збереження ліофілізованого матеріалу. У роботі проаналізовано дані щодо використання ліопротекторів та антиоксидантів в умовах ліофілізації та обговорюються питання безпеки використання методів доставлення ліопротекторів відносно геномного профілю клітин.

 

Probl Cryobiol Cryomed 2023; 33(4): 227–249

Біографії авторів

Anatoliy Goltsev, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

Olena Lutsenko, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

Lyudmyla Ostankova, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

Mykola Bondarovych, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

Maksym Ostankov, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

Larysa Sokil, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

Igor Hrisha, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

Lyudmyla Chernyshenko, Інститут проблем кріобіології і кріомедицини НАН України, м. Харків

Відділ кріопатофізіології і іммунології

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2024-03-10

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Goltsev, A., Lutsenko, O., Ostankova, L., Bondarovych, M., Ostankov, M., Sokil, L., Hrisha, I., & Chernyshenko, L. (2024). Сучасні підходи і перспективи методів ліофілізації ядерних клітин кордової крові людини. Проблеми кріобіології і кріомедицини, 33(4), 227–249. https://doi.org/10.15407/cryo33.04.227

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