Effect of Cryopreservation Conditions on Phenotypic Profile and Immunomodulating Activity of Fetal Liver Cells
DOI:
https://doi.org/10.15407/cryo29.03.266Keywords:
cryopreservation, DMSO, rat fetal liver cells, immunomodulating activity, experimental atopic dermatitisAbstract
The effect of cryopreservation conditions (concentration of dimethyl sulfoxide cryoprotector − DMSO, cooling rate in different temperature ranges) on phenotypic profile and immunomodulatory activity of rat fetal liver cells (FLC) of 14 days of gestation was investigated. The cells were frozen in two modes under the protection of 7.5; 10, and 12.5% DMSO. We evaluated the phenotypic profile of FLCs (CD34+CD38–, Sca-1+CD34-, Sca-1+CD34+, CD44-CD73+, CD44+CD73+), their cytomorphological indices, number and viability. The FLCs cryopreservation mode developed in this work ensured the preservation in a heterogeneous cell population with the immunomodulating activity, which was evaluated in the in vivo system on the model of experimental atopic dermatitis (EAD). The efficacy of using cryopreserved FLCs in mode 1 (1°С / min to –25°С; stabilization at –25°С for 5 min and plunging in liquid nitrogen) with 10% DMSO to restore blood parameters and reduce the intensity of skin inflammatory reactions in rats with induced EAD was experimentally established.
Probl Cryobiol Cryomed 2019; 29(3): 266–276.
References
Belous AM, Grishenko VI. [Cryobiology]. Kyiv: Naukova Dumka; 1994. 432 p. Russian.
Chinnici CM, Pietrosi G, Iannolo G, et al. Mesenchymal stromal cells isolated from human fetal liver release soluble factors with a potential role in liver tissue repair. Differentiation. 2019;105:14-26. CrossRef
Félix OMWO, Tunes G, Ginani VC, et al. The influence of cell concentration at cryopreservation on neutrophil engraftment after autologous peripheral blood stem cell transplantation. Hematol Transfus Cell Ther. 2018;40(3):233-9. CrossRef
Goltsev AN, Yampolskaya KY, Dubrava TG. [Identifi cation of fenotypical characteristics and an estimation of influence of various cryopreservation regimes on functional potential of fetal liver cells]. The Journal of V.N. Karazin Kharkiv National University. Series 'Biology' 2006; (4):110-20. Russian.
Goltsev AN, Dubrava TG, Ostankova LV, et al. Peculiarities of cryopreservation effect on functional potential of fetal liver hemopoietic stem cells of various gestation terms. Problems of Cryobiology. 2009;19(2): 186-99.
Goltsev AN, Bondarovich NA, Kuznyakov AV, et al. Determination of immunity T-cell link state and content of cancer stem cells as criterion to estimate efficiency of preventive breast cancer therapy with cryopreserved fetal liver cells. Probl Cryobiol Cryomed. 2014; 24(3):238-48. CrossRef
Goltsev AN, Gayevskaya YuA, Dubrava TG, Ostankova LV. Effect of cryopreservation on functional status of bone marrow hematopoietic and mesenchymal stem cells in animals with autoimmune pathology. Probl Cryobiol Cryomed. 2016; 26(1): 63-72. CrossRef
Gordienko EA, Pushkar NS. [Physical basis of low-temperature preservation of cell suspensions]. Kyiv: Naukova Dumka; 1994. 144p. Russian.
Grishchenko VI, Petrenko YuO, Skorobogatova NG, et al., inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of cryopreservation of hemoglobemic cells of human embryonic liver]. Patent of Ukraine № 67587A. 15.06.04. Ukrainian.
Grishchenko VI, Seminozhenko VP, Petrenko OYu, et al., inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of hematopoietic cells cryopreservation]. Patent of Ukraine № 58997A 15.08.03. Ukraine.
Grishenko VI, Goltsev A.N. Transplantation of the products of embrofetoplacental complex. From understanding of mechanism of the effect to increasing the efficiency of application. Problems of Cryobiology. 2002; 12(1): 54-84.
Hall SR, Jiang Y, Leary E, et al. Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometry. Stem Cells Transl Med. 2013; 2 (8):567-78. CrossRef
Humpe A, Beck C, Schoch R, et al. Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining. Transfusion. 2005; 45(7): 1208-13. CrossRef
Ikemoto J, Yoshihara S, Kobayashi T, et al. Aldehyde dehydrogenase activity in cryopreserved cord blood cells for quality assessment prior to transplantation. Mol Med Rep. 2018;18(5):4530-34. CrossRef
Morrison SJ, Hemmati HD, Wandycz AM, et al. The purification and characterization of fetal liver hematopoietic stem cells. Proc Natl Acad Sci USA. [Internet]. 1995; 92(22): 10302-6. [Cited 05.09.19]. Available from: https://www.pnas.org/content/pnas/92/22/10302.full.pdf. CrossRef
Naaldijk Y, Staude M, Fedorova V, Stolzing A. Effect of different freezing rates during cryopreservation of rat mesenchymal stem cells using combinations of hydroxyethyl starch and dimethylsulfoxide. BMC Biotechnol. [Internet]. 2012;12:49. [Cited 05.09.2019] Available from: https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-12-49. CrossRef
Nho SH, Yoon G, Seo JH, et al. Licochalcone H induces the apoptosis of human oral squamous cell carcinoma cells via regulation of matrin 3. Oncol Rep. 2019; 41(1):333-40.CrossRef
Orlic D, Porcellini A, Rizolli V. Electron microscopy of human fetal liver erythroid cells before and after cryopreservation. Exp Hematol. 1982; 1(7): 628-36.
Ostankov MV. Effect of rapid two-step freezing on bone marrow cell integrity. Problems of Cryobiology. 2007;17 (3):283-9.
Ruiz-Delgado GJ, Mancías-Guerra C, Tamez-Gómez EL, et al. Dimethyl sulfoxide-induced toxicity in cord blood stem cell transplantation: report of three cases and review of the literature. Acta Haematol. [Internet]. 2009; 122(1): 1-5. [Cited 05.09.2019]. Available from: https://www.karger.com/Article/Abstract/227267. CrossRef
Volkovа NA, Yukhta MS, Goltsev AN. Morphological and functional characteristics of cryopreserved multipotent mesenchymal stromal cells from bone marrow, adipose tissue and tendons. Cell and Organ Transplantology. 2016; 4(2): 200-5. CrossRef
Xu Y, Fan WW, Xu W, et al. Yiguanjian decoction enhances fetal liver stem/progenitor cell-mediated repair of liver cirrhosis through regulation of macrophage activation state. World J Gastroenterol. 2018;24(42):4759-72. CrossRef
Yampolskaya KY, Goltsev AN, Gurina TM. [Changes in the functional potential of fetal liver cells depending on the mode of cryopreservation]. World of Medicine and Biology. 2007; (1): 89-93. Russian.
Zalkan PM, Ivleva EA [Experimental model of allergic dermatitis]. In: Dolgov AP, Raben AS, Antonev AA, editors. [Topical issues of professional dermatology]. Moscow: Meditsina, 1965. p 106-12.
Zhang W, Hu J, Ma Q, et al. Cryopreserved mouse fetal liver stromal cells treated with mitomycin C are able to support the growth of human embryonic stem cells. Exp Ther Med. 2014;8(3):935-42. CrossRef
Published
How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
