Phase Transitions at the Temperatures Below 0°C in Mixtures of Cell Suspensions with Human Placental Extract Fractions

Authors

  • A. V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. S. Govorova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. N. Bobrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

placenta extract fractions, phase transitions, cell suspension, erythrocytes, Saccharomyces cerevisiae, eutectics, inversion, differential scanning calorimetry

Abstract

Using differential scanning calorimetry the phase transitions at the temperatures below 0°Cin fractions of extracts derived from human placenta with molecular mass up to 5; 50–70 and 750 kDa, as well as in suspensions of red blood cells and the yeasts Saccharomyces cerevisiae supplemented with the mentioned fractions of extracts were studied. General regularities of the influence of cells on low-temperature phase transitions in the fractions of extracts were revealed. A significant reduction in the intensities of the inversion peak and the eutectic melting peak was found likely due to the presence of NaCl. These phenomena are likely caused by the changed intermolecular interactions in the liquid phase as a result of competition between active centers of organic molecules for binding the water molecules. Reduced intensity of low-energy process of inversion, caused by conformational change in molecules of the fractions in the presence of cells, result from interactions of molecules involved in the process of inversion with the cells.

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Published

2024-05-21

How to Cite

Zinchenko, A. V., Govorova, Y. S., & Bobrova, E. N. (2024). Phase Transitions at the Temperatures Below 0°C in Mixtures of Cell Suspensions with Human Placental Extract Fractions. Problems of Cryobiology and Cryomedicine, 21(3), 314–320. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/129

Issue

Vol. 21 No. 3 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. V. Zinchenko, Yu. S. Govorova, E. N. Bobrova

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