Impact of Bacterization with Anabaena flos-aquae and Pseudomonas putida and Salicylic Acid Treatment on Cold Resistance in Leguminous Plants
Keywords:
bacterization, salicylic acid, cold stress, cold resistance of plants, Pisum sativum, Phaseolus vulgarisAbstract
The paper presents the results of studying the eff ects of exposure of pea (Pisum sativum) and been (Phaseolus vulgaris) seeds or young sprouts to low temperature (4°C) with preliminary treatment of seeds with salicylic acid (SA), or with pure cultures of Anabaena flos-aquae or Pseudomonas putida, or with their mixture. Cold exposure lasting 24 hrs was carried out immediately or 7 days after SA treatment or bacterization of seeds. Morphometric parameters of roots and sprouts, permeability of cell membranes in leaves, activity of polyphenol oxidase and state of actin filaments in root cells were measured. Pea and bean plants differ in cold resistance, and their growth responses to low temperature exposure were multidirectional. Treatment with SА immediately before exposure to cold had a positive effect on cold resistance of pea plants. Under the delayed cold stress, seed bacterization with single bacteria suspensions or their mixture contributed to the development of cold resistance in beans. Bacterization of seeds presumably reduced the permeability of cell membranes in leaves of both plant species and improved the state of actin filaments in root cells. In general, some protective effect for peas was observed due to either bacterial or SА treatment of seeds. In bean plants, the meaningful protective effect occurred after seed bacterization only.
Probl Cryobiol Cryomed 2024; 34(2):125–142
References
Abo-Shady AM, Osman MEH, Gaafar RM, et al. Cyanobacteria as a valuable natural resource for improved agriculture, environment, and plant protection. Water Air Soil Pollut. 2023 May 4 [cited 2024 Jul 24]; 234(5): 313. Available from: https://link.springer.com/article/10.1007/s11270-023-06331-7 CrossRef
Acuña-Rodríguez IS, Newsham KK, Gundel PE, et al. Functional roles of microbial symbionts in plant cold tolerance. Ecol Lett. 2020; 23(6): 1034-48. CrossRef
Alvarez AL, Weyers SL, Goemann HM, Peyton BM, Gardner RD. Microalgae, soil and plants: A critical review of microalgae as renewable resources for agriculture. Algal Research. 2021 Feb 9 [cited 2024 Jul 24]; 54: 102200. Available from: https://www.sciencedirect.com/science/article/abs/pii/S2211926421000199 CrossRef
Atramentova LO, Utevska OM. [Statistical methods in biology]. Kharkiv: V.N. Karazin Kharkiv National University; 2007. 288 p. Ukrainian.
Bhat KA, Mahajan R, Pakhtoon MM, et al. Low temperature stress tolerance: An insight into the omics approaches for legume crops. Front Plant Sci. 2022 Jun 3 [cited 2024 Jul 24]; 13: 888710. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204169/ CrossRef
Chauhan M, Kimothi A, Sharma A, et al. Cold adapted Pseudomonas: ecology to biotechnology. Front Microbiol. 2023 Jul 17 [cited 2024 Jul 24]; 14: 1218708. Available from: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1218708/full CrossRef
Deshmukh AJ, Jaiman RS, Bambharolia RP, et al. Seed biopriming-a review. Int J Econ Plants. 2020; 7(1): 38-43. CrossRef
Hasanuzzaman M, Fujita M, Oku H, Islam MT, editors. Plant tolerance to environmental stress: Role of phytoprotectants. Boca Raton: CRC Press; 2019. 468 p. CrossRef
Jankovska-Bortkevič E, Katerova Z, Todorova D, et al. Effects of auxin-type plant growth regulators and cold stress on the endogenous polyamines in pea plants. Horticulturae. 2023 Feb 10 [cited 2024 Jul 24]; 9(2): 244. Available from: https://www.mdpi.com/2311-7524/9/2/244 CrossRef
Kazemi-Shahandashti SS, Maali-Amiri R. Global insights of protein responses to cold stress in plants: Signaling, defence, and degradation. J Plant Physiol. 2018; 226: 123-35. CrossRef
Keskin SO, Ali TM, Ahmed J, et al. Physico‐chemical and functional properties of legume protein, starch, and dietary fiber-A review. Legume Science. 2022 Mar 16 [cited 2024 Jul 24]; 4(1): e117. Available from: https://onlinelibrary.wiley.com/doi/10.1002/leg3.117 CrossRef
Kollmen J, Strieth D. The beneficial effects of cyanobacterial co-culture on plant growth. Life (Basel). 2022 Jan 31 [cited 2024 Jul 24]; 12(2): 223. Available from: https://www.mdpi.com/2075-1729/12/2/223 CrossRef
Kolupaev YuE, Yastreb TO, Shkliarevskyi MA, et al. [Salicylic acid: synthesis and stress-protective effects in plants]. The bulletin of Kharkiv national agrarian university. Series biology. 2021; (2): 6-22. Ukrainian. CrossRef
Koo YM, Heo AY, Choi HW. Salicylic acid as a safe plant protector and growth regulator. Plant Pathol J. 2020; 36(1): 1-10. CrossRef
Kumar M, Poonam Ahmad S, Singh RP. Plant growth promoting microbes: Diverse roles for sustainable and ecofriendly agriculture. Energy Nexus. 2022 Sep 1 [cited 2024 Jul 24]; 7: 100133. Available from: https://www.sciencedirect.com/science/article/pii/S2772427122000882 CrossRef
Kumar S, Jeevaraj T, Yunus MH, et al. The plant cytoskeleton takes center stage in abiotic stress responses and resilience. Plant Cell Environ. 2023; 46(1): 5-22. CrossRef
Kushwaha P, Kashyap PL, Kuppusamy P. Microbes for cold stress resistance in plants: mechanism, opportunities, and challenges. In: Goel R, Soni R, Suyal D, editors. Microbiological Advancements for Higher Altitude Agro-Ecosystems and Sustainability. (Rhizosphere Biology). Singapore: Springer; 2020. p. 269-92. CrossRef
Lone AA, Khan MN, Gul A, et al. Common beans and abiotic stress challenges. Curr J Appl Sci Technol. 2021; 40(14): 41-53. CrossRef
Ma H, Liu M. The microtubule cytoskeleton acts as a sensor for stress response signaling in plants. Mol Biol Rep. 2019; 46(5): 5603-8. CrossRef
Mishra PK, Bisht SC, Ruwari P, et al. Alleviation of cold stress in inoculated wheat (Triticum aestivum L.) seedlings with psychrotolerant Pseudomonas from NW Himalayas. Arch Microbiol. 2011; 193(7): 497-513. CrossRef
Morcillo RJL, Manzanera M. The effects of plant-associated bacterial exopolysaccharides on plant abiotic stress tolerance. Metabolites. 2021 May 24 [cited 2024 Jul 24]; 11(6): 337. Available from: https://www.mdpi.com/2218-1989/11/6/337 CrossRef
Qian D, Xiang Y. Actin cytoskeleton as actor in upstream and downstream of calcium signaling in plant cells. Int J Mol Sci. 2019 Mar 20 [cited 2024 Jul 24]; 20(6): 1403. Available from: https://www.mdpi.com/1422-0067/20/6/1403 CrossRef
Rais A, Jabeen Z, Shair F, et al. Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLoS One. 2017 Nov 21 [cited 2024 Jul 24]; 12(11): e0187412. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187412 CrossRef
Rawat N, Singla-Pareek SL, Pareek A. Membrane dynamics during individual and combined abiotic stresses in plants and tools to study the same. Physiol Plant. 2021; 171(4): 653-76. CrossRef
Saijo Y, Loo EP. Plant immunity in signal integration between biotic and abiotic stress responses. New Phytol. 2020; 225(1): 87-104. CrossRef
Shevchuk OA, Kravchuk GI, Vergelis VI, et al. [The effect of stimulant drugs on the morphometric indicators of seedlings and sowing qualities of bean seeds]. Agriculture and forestry. 2019; (12): 225-32. Ukrainian.
Solomon W, Mutum L, Janda T, et al. Potential benefit of microalgae and their interaction with bacteria to sustainable crop production. Plant Growth Regul. 2023; 101: 53-65. CrossRef
Soualiou S, Duan F, Li X, et al. Crop production under cold stress: An understanding of plant responses, acclimation processes, and management strategies. Plant Physiol Biochem. 2022; 190: 47-61. CrossRef
Sun D, Zhuo T, Hu X, et al. Identification of a Pseudomonas putida as biocontrol agent for tomato bacterial wilt disease. Biological Control. 2017; 114: 45-50. CrossRef
Takács G, Stirk WA, Gergely I, et al. Biostimulating effects of the cyanobacterium Nostoc piscinale on winter wheat in field experiments. S Afr J Bot. 2019; 126: 99-106. CrossRef
Zboralski A, Filion M. Pseudomonas spp. can help plants face climate change. Front Microbiol. 2023 Jun 23 [cited 2024 Jul 24]; 14: 1198131. Available from: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1198131/full CrossRef
Zhang S. Recent advances of polyphenol oxidases in plants. Molecules. 2023 Feb 25 [cited 2024 Jul 24]; 28(5): 2158. Available from: https://www.mdpi.com/1420-3049/28/5/2158 CrossRef
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
