КриоÑÐ»ÐµÐºÑ‚Ñ€Ð¾Ð½Ð½Ð°Ñ Ð¼Ð¸ÐºÑ€Ð¾ÑÐºÐ¾Ð¿Ð¸Ñ ÐºÐ°Ðº инÑтрумент ÑиÑтемной биологии, Ñтруктурного анализа и ÑкÑпериментального воздейÑÑ‚Ð²Ð¸Ñ Ð½Ð° клетки. КомплекÑный аналитичеÑкий обзор новейших работ
DOI:
https://doi.org/10.15407/cryo24.03.193Ключевые слова:
криоÑÐ»ÐµÐºÑ‚Ñ€Ð¾Ð½Ð½Ð°Ñ Ð¼Ð¸ÐºÑ€Ð¾ÑкопиÑ, криоÑÐ»ÐµÐºÑ‚Ñ€Ð¾Ð½Ð½Ð°Ñ Ñ‚Ð¾Ð¼Ð¾Ð³Ñ€Ð°Ñ„Ð¸Ñ, ÑиÑÑ‚ÐµÐ¼Ð½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ, локаломика, динамомика, Ñтруктурный анализ, in silico, микромашинингАннотация
Целью наÑтоÑщей работы ÑвлÑетÑÑ Ð´ÐµÐ¼Ð¾Ð½ÑÑ‚Ñ€Ð°Ñ†Ð¸Ñ Ð½Ð¾Ð²Ð¾Ð³Ð¾ взглÑда на криоÑлектронную микроÑкопию биологичеÑких Ñтруктур как на проÑтой многофакторный инÑтрумент ÑиÑтемной биологии, допуÑкающий применение его длÑультраÑтруктурного позиционно-чувÑтвительного и динамичеÑкого анализа в молекулÑрной цитоморфологии и Ñмежных диÑциплинах. РаÑÑматриваютÑÑ Ñ€Ð°Ð·Ð»Ð¸Ñ‡Ð½Ñ‹Ðµ методы криоÑлектронной аналитики и ÑоответÑтвующие ÑиÑтемно-
биологичеÑкие интерпретации, ÑиÑÑ‚ÐµÐ¼Ð°Ñ‚Ð¸Ð·Ð°Ñ†Ð¸Ñ ÐºÐ¾Ñ‚Ð¾Ñ€Ñ‹Ñ… может быть полезна Ð´Ð»Ñ Ð½Ð°Ñ‡Ð°Ð»ÑŒÐ½Ð¾Ð¹ поÑтановки прогреÑÑивных задач при иÑÑледованиÑÑ… данными методами и поÑледующей обработки полученных результатов. Показано, что ÑиÑтемноÑÑ‚ÑŒ методологии криоÑлектронной микроÑкопии напрÑмую корреÑпондирует Ñ ÑиÑтемноÑтью и многопараметричеÑким характером её аналитов в рамках ÑиÑтемной биологии. ПриводÑÑ‚ÑÑ Ð´Ð°Ð½Ð½Ñ‹Ðµ по иÑпользованию криоÑлектронной микроÑкопии в микроÑтруктурной обработке и микроманипулÑциÑÑ… на клетках (Ñ‚. н. micromachining), а также в разработке молекулÑрных
машин. Приведенные в наÑтоÑщем аналитичеÑком обзоре ÑÐ²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¾Ñнованы на данных криоÑлектронной микроÑкопии поÑледних лет.
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