КриоÑÐ»ÐµÐºÑ‚Ñ€Ð¾Ð½Ð½Ð°Ñ Ð¼Ð¸ÐºÑ€Ð¾ÑÐºÐ¾Ð¿Ð¸Ñ ÐºÐ°Ðº инÑтрумент ÑиÑтемной биологии, Ñтруктурного анализа и ÑкÑпериментального воздейÑÑ‚Ð²Ð¸Ñ Ð½Ð° клетки. КомплекÑный аналитичеÑкий обзор новейших работ
DOI:
https://doi.org/10.15407/cryo24.03.193Ключові слова:
кріоелектронна мікроÑкопіÑ, кріоелектронна томографіÑ, ÑиÑтемна біологіÑ, локаломіка, динаміка, Ñтруктурний аналіз, in silico, мікромашинінгАнотація
Метою даної роботи Ñ” демонÑÑ‚Ñ€Ð°Ñ†Ñ–Ñ Ð½Ð¾Ð²Ð¾Ð³Ð¾ поглÑду на кріоелектронну мікроÑкопію біологічних Ñтруктур Ñк напроÑтий багатофакторний інÑтрумент ÑиÑтемної біології, Ñкий допуÑкає заÑтоÑÑƒÐ²Ð°Ð½Ð½Ñ Ð¹Ð¾Ð³Ð¾ Ð´Ð»Ñ ÑƒÐ»ÑŒÑ‚Ñ€Ð°Ñтруктурного позиційно-чутливого, а також динамічного аналізу в молекулÑрній цитоморфології та Ñуміжних диÑциплінах. РозглÑдаютьÑÑ Ñ€Ñ–Ð·Ð½Ñ– методи кріоелектронної аналітики та відповідні ÑиÑтемно-біологічні інтерпретації, ÑиÑÑ‚ÐµÐ¼Ð°Ñ‚Ð¸Ð·Ð°Ñ†Ñ–Ñ Ñких може бути кориÑною Ð´Ð»Ñ Ð¿Ð¾Ñ‡Ð°Ñ‚ÐºÐ¾Ð²Ð¾Ñ— поÑтановки прогреÑивних задач під Ñ‡Ð°Ñ Ð´Ð¾Ñліджень цими методами та наÑтупної обробки отриманих результатів. Показано, що ÑиÑтемніÑÑ‚ÑŒ методології кріоелектронної мікроÑкопії прÑмо кореÑпондує зі ÑиÑтемніÑÑ‚ÑŽ та багатопараметричним
характером Ñ—Ñ— аналітів у рамках ÑиÑтемної біології. Ðаведено дані щодо викориÑÑ‚Ð°Ð½Ð½Ñ ÐºÑ€Ñ–Ð¾ÐµÐ»ÐµÐºÑ‚Ñ€Ð¾Ð½Ð½Ð¾Ñ— мікроÑкопії у мікро-
Ñтруктурній обробці та мікроманіпулÑціÑÑ… на клітинах (Ñ‚. зв. micromachining), а також у розробці молекулÑрних машин. У
цьому аналітичному обзорі наведено відомоÑÑ‚Ñ– на оÑнові даних кріоелектронної мікроÑкопії оÑтанніх років.
Посилання
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