Effect of Oxyethylated Glycerol with Polymerization Degree n = 5 on Human Hemoglobin Thermostability

Authors

  • Aleksandra V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yulianna S. Govorova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Antonina M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

melting, hemoglobin, thermal stability, oxyethylated derivative of glycerol with polymerization degree n = 5, differential adiabatic scanning microcalorimetry

Abstract

The effect of oxyethylated derivative of glycerol with polymerization degree n = 5 (OEGn=5) on thermodynamic and kinetic parameters of hemoglobin melting was studied by the method of differential adiabatic scanning microcalorimetry. It was shown that increase of OEGn=5 concentration in solution resulted in reduction of the temperature and calorimetric enthalpy of hemoglobin thermodenaturation. It was suggested that the increasing of cryoprotective concentration induced the loosening of hemoglobin molecules thereby contributing to the reduction of thermal stability of protein. The possibility of using kinetic approach when analyzing hemoglobin melting process was shown. Probl Cryobiol Cryomed 2013; 23(2):135-142.

Author Biographies

Aleksandra V. Zinchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Yulianna S. Govorova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Antonina M. Kompaniets, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryoprotectants

References

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Published

2013-06-20

How to Cite

Zinchenko, A. V., Govorova, Y. S., & Kompaniets, A. M. (2013). Effect of Oxyethylated Glycerol with Polymerization Degree n = 5 on Human Hemoglobin Thermostability. Problems of Cryobiology and Cryomedicine, 22(2), 135–142. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/30

Issue

Vol. 22 No. 2 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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Copyright (c) 2020 Aleksandra V. Zinchenko, Yulianna S. Govorova, Antonina M. Kompaniets

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