Effect of Intracellular Trehalose on Post-Thaw Survival of Saccharomyces cerevisiae Yeast Cells

Authors

  • Viktoria L. Ponomareva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Igor P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Elena S. Onasenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo24.03.268

Keywords:

yeast, cryopreservation, immobilization, trehalose, viability

Abstract

N/A Probl Cryobiol Cryomed 2014; 24(3):268-272.

Author Biographies

Viktoria L. Ponomareva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long Term Storage of Biological Objects at Low Temperatures

Igor P. Vysekantsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long Term Storage of Biological Objects at Low Temperatures

Elena S. Onasenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long Term Storage of Biological Objects at Low Temperatures

References

de Antoni G.L., Perez P., Abraham A. et al. Trehalose, a cryoprotectant for Lactobacillus bulgaricus. Cryobiology. – 1989. – Vol.26. – P. 149–153.

Diniz-Mendes L., Bernandes E., de Araujo P.S. et al. Preservation of frozen yeast cells by trehalose. Biotechnol Bioeng 1999; 65(5): 572–578. CrossRef

Glantz S.A. Primer of biostatistics. New York: McGraw Hill; 2004.

Lusta K.A., Fikhte B.A., editors. Methods for determination of microorganism viability. Puschino; 1990.

Nakamura T., Takagi H., Shima J. Effects of ice-seeding temperature and intracellular tregalose contents on survival of frozen Saccharomyces cerevisiaе cells. Cryobiology 2009; 58: 170–174. CrossRef PubMed

Netrusov A.I., editor. Course in microbiology. Moscow: Akademiya; 2005.

Panek AD. Trehalose metabolism – new horizons in technological applications. Braz J Med Biol Res 1995; 28(2): 169–181.

Ponomareva V.L., Vysekantsev I.P., Gurina T.M. et al. Study of cryopreservation effect on viability of Saccharomyces cerevisiae yeast cells immobilized in alginate gel. Visnyk Problem Biologii i Medytsyny 2012; 1(92): 14-17.

Ponomareva V.L., Vysekantsev I.P., Onasenko E.S. et al. Functional activity of cryopreserved Saccharomyces cerevisiae yeast cells immobilized in alginate gel. Proceedings of the International Scientific-Practical Conference 'Theoretical and Practical Aspects of Modern Cryobiology'; Syktyvkar; 2014; p. 214–219.

Sarishvili N.G., editor. Microbiological basics of champagne technology. Moscow: Pischevaya Promyshlennost; 2000.

Sinitsin A.P., Raynina E.I., Lozinsky V.I., Spasov S.D., editors. Immobilized cells of microorganisms. Moscow: Moscow State University; 1994.

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Published

2014-09-15

How to Cite

Ponomareva, V. L., Vysekantsev, I. P., & Onasenko, E. S. (2014). Effect of Intracellular Trehalose on Post-Thaw Survival of Saccharomyces cerevisiae Yeast Cells. Problems of Cryobiology and Cryomedicine, 24(3), 268–272. https://doi.org/10.15407/cryo24.03.268

Issue

Vol. 24 No. 3 (2014): Problems of Cryobiology and Cryomedicine

Section

Short communications

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Copyright (c) 2020 Viktoria L. Ponomareva, Igor P. Vysekantsev, Elena S. Onasenko

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